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KMID : 0357920100440020191
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Korean Journal of Pathology
2010 Volume.44 No. 2 p.191 ~ p.198
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Galectin-3 Expression and BRAF Mutation in Cases of Cytologically Suspicious Papillary Thyroid Carcinoma
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Kim Do-Kyung
Kim Hyun-Gi
Kwak Jin-Young
Kim Min-Ju
Jung Hyung-Jae
Koo Ja-Seung
Lim Beom-Jin
Jung Chan-Kwon
Hong Soon-Won
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Abstract
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Background: Fine needle aspiration, which is known as the most accurate and cost-effective method for diagnosis of thyroid nodule, still may result in indeterminate cases that are pauci-cellular and show minor nuclear atypia, but most cases are associated with suspicion of papillary thyroid carcinoma (PTC). A B-type Raf kinase (BRAF) mutation was found in about half of PTCs and galectin-3 was expressed by malignant tumors, helping us to differentiate malignancies from benign lesions.
Methods: Cases studied included histologically 44 confirmed PTC cases and 18 benign cases previously diagnosed as suspicious of PTC using cytologic examination. Cases were analyzed for galectin-3 expression by immunohistochemical staining and BRAF mutation by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) with a new restriction enzyme.
Results: All 44 cases of PTC and 8 of 18 benign controls expressed galectin-3. BRAF mutations were found in only 9 of the 44 PTC cases. Assessment of galectin-3 expression demonstrated high sensitivity but low specificity. Evaluation of BRAF mutation revealed high specificity and low sensitivity.
Conclusions: This study suggests that the combined application of these two methods for PTC of suspicious cytology is complementary.
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KEYWORD
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Suspicious cytology, Biopsy, fine needle, BRAF mutation, Galectin-3
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